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پیوست 1: محلولها و بافرهاي مورد نياز جهت استخراج DNA
1-1- ترکیب محلولهاي مورد نياز براي استخراج DNA پلاسميدي در مقياس کم به روش ليز قليايي به شرح زیر میباشد.
حجم مورد استفاده
غلظت نهایی
ترکیبات
بافر
1.25 ml
1 ml
5 ml
42.75 ml
25 mM
10mM
50mM
Tris – HCl 1M pH:8
EDTA 0.5 M pH:8
Glucoses 0.5 M
ddH2O
Solution I
(50 ml)
0.5 ml
2.5 ml
22

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